Composite

Part:BBa_K146001

Designed by: Dave Westenberg   Group: iGEM08_Missouri_Miners   (2008-10-29)


AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

This part is to be constructed by cloning part BBa_I764001 into the RFP vector BBa_J61002. Vector and insert are to be each digested with EcoRI and SpeI and ligated together to create the new composite part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3802
    Illegal XbaI site found at 3817
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3802
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
    Illegal NotI site found at 1753
    Illegal NotI site found at 3808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3802
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3802
    Illegal XbaI site found at 3817
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3802
    Illegal XbaI site found at 3817
    Illegal SpeI site found at 877
    Illegal PstI site found at 1760
    Illegal AgeI site found at 1456
    Illegal AgeI site found at 1568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2841


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Parameters
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